In the era of functional genomics studies of microbial physiology present a crutial source of information since not all responds of cells toward the changes in the medium could be observed in spite of the use of sofisticated modern techniques. Under specific growth conditions such changes could be easily detected by following the development of the cells through time and taking into account morphological diferenciations as well. Such approach enabled us to detect the post-translational modification of 6-phosphofructo-1-kinase (PFK) in Aspergillus niger cells, that takes place spontaneously by the induced activity of intracellular proteases. Phosphorylation of the protein molecule was found to be neccessary for reactivation of the fragmented enzyme. Fragmented protein showed changed kinetic parameters in comparison to the native protein. It is better induced by fructose-2,6-biphosphate and it is resistant to inhibition by citrate. Deregulated enzyme excelerates glycolitic flux through glycolysis and significantly contributes to the higher rate of product formation. Truncated gene that would code for synthesis of the fragmented PFK will be transformed into other commercial organisms where increased glycolytic flux and higher productivity is expected. A transfer of specific key genes from Aspergillus niger into other micoorganisms of commercial interest is a subject of international project co-financed by EU Commission under the scope of 5. Framework programme. The project is co-ordinated by dr. Matic Legiša.