gies, this approach does not allow preservation of female genetic material. Recently, transplantation of spermatogonia and oogonia into suitable recipients after cryopreservation was developed, enabling preservation of both male and female genetic material. In this study, two experiments were designed to test the appropriateness of different cryoprotectants and their concentrations for slow-rate freezing of brown trout juvenile gonadal tissue. Juvenile testes (∼15 mg) and ovarian tissue (∼25 mg) were excised and equilibrated in cryomedia for 1 hour on ice. Samples were then cryopreserved in 2 ml cryotubes at 1 °C/min freezing rate to -−80˚C followed by a plunge to liquid nitrogen. After thawing at 10 °C, tissue was dissociated. Viability of spermatogonia and oogonia was checked by trypan blue staining and calculated as the proportion of viable cells number after cryopreservation compared to the number of viable fresh cells. Samples of both tissues had a significantly higher viability when cryopreserved with Me2SO comparing to all other cryoprotectants. Present results suggest that 1.6 M Me2SO is suitable for preservation of brown trout gonadal tissue.
B.03 Paper at an international scientific conference
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