Analysis of required oligonucleotide motives of TLR9 agonists showed that minimal required motifs activating mouse TLR9 differ from that required for activation of human TLR9. We showed that only oligonucleotides with two spatially positioned CpG motives could activate the human antigen presenting cells like plasmacytoid dendritic cells and B-cells. Moreover, we showed that these stringent requirements for the synthetic agonists are consequences of the three amino acid residues within human TLR9.
COBISS.SI-ID: 6089754
In the present study, we extrapolate the structure of flagellin-TLR5 complex using structure-guided mutagenesis to determine the recognition modes of human and mouse TLR5 receptors and demonstrate species-specific differences in flagellin recognition. In general, the recognition mode of the mouse receptor can be said to be more robust in comparison to that of the human receptor.
COBISS.SI-ID: 5948186