The present European patent (EP2662691) relates to methods and materials for the diagnosis of anaphylaxis, and more particularly to the role of basophil absolute count and the expression of the high affinity IgE in the occurrence of anaphylaxis. Methods of diagnosing anaphylaxis by determining the absolute number of basophils in whole blood or by determining the expression of the [alpha]-chain of high affinity IgE receptor in whole blood are disclosed. An important part of this new methodology was developed during the Slovenian Research Agency's project Evaluations of patients complete specific IgEs allergenic activity by combination of recombinant allergens and multiplex basophil cellular approach.
F.32 International patent
COBISS.SI-ID: 2048258673The project leader prof. dr. Peter Korošec has become an Editorial Board Member of Clinical and Experimental Allergy in 2015. This journal's impact factor is 5.264 (2016) and is ranking 4th place in the field of Allergy.
C.06 Editorial board membership
COBISS.SI-ID: 26785280Prof. dr. Peter Korošec was a mentor to Julij Šelb for his PhD thesis Integrated diagnostic evaluation of Hymenoptera venom allergic patient. In this PhD thesis we wanted to evaluated novel diagnostic approaches in Hymenoptera venom allergy with the focus on the venom recombinant allergens and basophil activation test. We showed that the combination of recombinant allergens and multiplex activation of the basophil test is the most accurate approach for diagnosis of complex cases and that this approach has a high clinical value.
D.09 Tutoring for postgraduate students
COBISS.SI-ID: 289594624Prof. dr. Peter Korošec was a co-mentor to Jernej Luzar for his PhD thesis Identification and characterization of allergens Fel d 1 and Ara h 2 mimotopes. In this PhD thesis we evaluated the epitop function of two major allergens by mimotop experimental approaches and evaluated the IgE allergogenic activity. We showed that Fel d 1 epitops have comformational structure and Ara h 2 epitops linear structure. Those epitops were not able to induce allergogenic IgE activity (they not cross-link IgEs on the surface of effector cells), despite, high IgE binding activity. Those results are very important for the novel diagnostic and therapeutic approaches in allergic diseases.
D.09 Tutoring for postgraduate students
COBISS.SI-ID: 4264049Prof. dr. Peter Korošec was mentor to Matjaž Homšak for his PhD thesis Importance of peanut recombinant allergens and basophil activation test in peanut sensitivity. In this PhD thesis we showed that high basophil sensitivity in BAT test is associated with anaphylactic reactions. Low CD-sens levels exclude the possibility of anaphylaxis or even peanut allergy. Peanut recombinant allergens are important in peanut allergy. Especially rAra h 2 express important allergenicity and is related with major clinical symptoms. Higher rAra h 2 levels and additional peanut recombinant allergens rAra h1 and h 3 increase the likelihood of peanut allergy. rAra h 9 showed no allergenicity in our population. Homologous allergens Ara h 8 and Bet v 1 are important in birch allergy. If rAra h 2 concentration was ) 0,35 KE/L then peanut allergy is suggested. In case of additionally elevated concentrations of rAra h 1 and h 3 we can avoid additional peanut challenge test in 85 %, what we can consider in diagnostic algorithm. Almost completely we can avoid peanut challenge in case of negative BAT testing results.
D.09 Tutoring for postgraduate students
COBISS.SI-ID: 512499768