We presented our results at the Slovenian Biochemical Society meeting. Synthetic scaffolds proved to be successful tool for improving biosynthetic pathway efficiency. Our aim was to test the activity of large multi-enzymatic complexes where clustering was mediated by polypeptide elements forming ordered structures. Complexes were produced in Escherichia coli and tested in small scale fermentation bioprocess. Multi-enzymatic particles were purified in very simple isolation process that includes only cellular lysis, centrifugation and final washing and tested in vitro in the production process. Reaction efficiencies of multi-enzymatic particles were compared to reaction systems where soluble enzymes were not linked together or to reaction systems composed of otherwise linked enzymes but not integrated into large subcellular structures.
F.02 Acquisition of new scientific knowledge
COBISS.SI-ID: 5778970At the meeting the results from our research in the field of designed protein origami, e.g. designed self-assembled polypeptide nanostructures from a single chain, were presented.
B.04 Guest lecture
COBISS.SI-ID: 5833242