In the frame of invited lecture on Standing Committee on Plants, Animals, Food and Feed, Section Plant Health in Brussels (DG SANCO) we present Pest risk analysis for Hop stunt viroid (HSVd) and Citrus bark cracking viroid (CBCVd). The presentation include results from the project and epidemiological characteristics of both viroids which are important for defining of viroids status and in making decisions for management. The presentation have great importance on decision of performing systematic monitoring on HSVd and CBCVd in member states.
B.04 Guest lecture
COBISS.SI-ID: 724108To support farmers and advisory services we prepared a professional manual which contain basic information on severe hop stunt disease, recognizing, and disease measures which are crucial for eradication from Slovene hop gardens. The manual is written based on experiences from other similar diseases, and results of our own research studies which were performed in Slovenian Institute of Hop Research and Brewing (IHPS) and on Biotechnical Faculty in Ljubljana. The manual is public accessible on web page of Administration of the Republic of Slovenia for Food Safety, Veterinary Sector and Plant Protection and on web page of IHPS. http://www.ihps.si/wp-content/uploads/2016/08/Huda-viroidna-zakrnelost-hmelja-2017.compressed.pdf.
F.17 Transfer of existing technologies, know-how, methods and procedures into practice
COBISS.SI-ID: 288782592In 10 years breeding process which includes many selection methods, a new variety named Styrian Wolf was released to the hop production. The variety have high yield, express high resistance to foliar diseases and have unique etheric oils which are important for craft beer production. The variety is popular among hop growers and as well among brewers what resulted in 40 ha of planted hop gardens in Slovenia. The variety was tested to CBCVd resistance in the frame of the project. The results revealed low level of CBCVd infections and high tolerance.
F.06 Development of a new product
COBISS.SI-ID: 726412Artificial plant inoculation methods are an important tool in plant virology and can be used in various studies, such as resistance screening, biological indexing of host plants, epidemiological studies and analysis of plant-pathogen interactions. With the aim of developing a reliable bioassay for CBCVd for hop genotype resistance screening and analysis of the CBCVd host range, we compared four different types of inoculum based on RNA and sap extract obtained from CBCVd infected donor plants. The inoculum was mechanically introduced using stem injections or rubbing the inoculum onto leaves treated with carborundum. The assay was performed on six indicator plants: two varieties of cucumber (Cucumis sativus), two varieties of tomato (Solanum lycopersicum), eggplant (Solanum melongena) and purple passion plant (Gynura aurantiaca), with 10 repetitive plants per treatment. The results of our preliminary study show that the best bioassay for CBCVd is rubbing the sap extract inoculum onto leaves treated with carborundum, using Heinz 1370 tomato variety as an indicator plant. Using inoculum based on sap extract, we were able to infect both varieties of cucumber and tomato, as well as eggplant.
F.17 Transfer of existing technologies, know-how, methods and procedures into practice
COBISS.SI-ID: 8301433The most commonly used methods for the detection of viroids, such as molecular hybridization and RT-PCR, are based on nucleic acids. In the case of aggressive hop stunt disease present in Slovenia, the presence of three different viroids on the diseased plants leads to higher costs of diagnosis and indicates the need for methods that allow simultaneous detection of all viroids. For this purpose, we have developed the diagnostic method mRT-PCR for detection of all four hop viroids. Use of mRT-PCR enables shorter analysis, reduces the use of reagents and, consequently, the cost of analysis. The method can be used for routine testing of symptomatic plants from a field, for testing in the context of a certification scheme for planting material, as well as for epidemiological studies.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 8259705