The Vjetrenica cave in the Dinaric Karst hosts a worldwide extraordinarily high cave biodiversity including, sprout-like formations attached to the bed of the cave stream. We investigated the sprout-like microbial aggregates by the rRNA approach and detailed microscopy. The aggregates are morphologically highly organized, and their core consists of a member classified as Candidatus Troglogloea absoloni. The surface of the sprout-like aggregates is more diverse than the core. It is colonized by a bacterial biofilm consisting primarily of filamentous Betaproteobacteria, whereas other microbial populations present inthe crust include members of the Bacteriodetes, Gammaproteobacteria, Actinombacteria, Alphaproteobacteria, and Planctomycetes, which are intermingled with mineral inclusions.
COBISS.SI-ID: 2711375
The evolution of antibiotic resistance could be prevented via inhibition of the interaction of the two principal regulators of the SOS response, the proteins LexA and RecA. To engineer inhibitors of their interaction, a 3D model was prepared showing that the N and C terminal domains of LexA interact with the activated RecA protein - RecA*.
COBISS.SI-ID: 26953767
While the C terminal domain of the uropathogenic specific protein Usp is homologous to colicin E7 it nevertheless enters eukaryotic cells and induces genome fragmentation as well as apoptosis.
COBISS.SI-ID: 2871119
A bacterial strain designated JA-1, related to Janthinobacterium lividum, was isolated from glacier ice samples from the island Spitsbergen in the Arctic. The strain was tested for phenotypic traits and the most prominent appeared to be the dark red brown to black pigmentation different from the violet pigment of Janthinobacterium, Chromobacterium and Iodobacter. Phylogenetic analysis based on 16S rRNA gene sequences and DNA-DNA hybridization tests showed that strain JA-1 belongs to the genus Janthinobacterium but represents a novel lineage. It is proposed as the type strain of a novel species of the genus Janthinobacterium, for which the name Janthinobacterium svalbardensis sp. nov. is proposed.
COBISS.SI-ID: 3147912
Transcriptome analysis revealed that exposure to subinhibitory concentrations of colicin M altered expression of genes involved in envelope, osmotic and other stresses, including genes of the CreBC two-component system,exopolysaccharide production and cell motility. Nonetheless, there was no induction of biofilm formation or genes involved in mutagenesis that can lead to evolution of antibiotic resistance, providing support for colicin M as a promising antimicrobial agent.
COBISS.SI-ID: 2748239