We synthesized a new lanthanide single thiol reacting tag for the paramagnetic labelling of proteins, named CysPhTAHA, which gives NMR spectra with a single set of peaks. Bound to ubiquitin, it induced large residual dipolar couplings and pseudocontact shifts that could be measured easily and agreed very well with the protein structure. We show that CysPhTAHA can be used to label large proteins that are biochemically challenging such as the Lac repressor in a 90 kDa ternary complex with DNA and inducer.
COBISS.SI-ID: 25010983
In this invited lecture our recent results of the nature of sPLA2-CaM interaction, were presented.
COBISS.SI-ID: 26807847
On the poster our most recent results of the nature of sPLA2-CaM interaction and the inhibitor that prevents the interaction, were presented.
COBISS.SI-ID: 36989189
On the poster our first NMR results of the nature of sPLA2-CaM interaction, were presented.
COBISS.SI-ID: 26286375
Bacterial SOS response play crucial rule in the development of bacterial antibiotic resistance, where a key regulator is a complex formed between single stranded DNA (ssDNA) and two proteins, RecA and LexA. Based on the experimental data we constructed three-dimensional model of a ssDNA-RecA-LexA complex, which offers new possibilities to fight antibiotic resistance of bacteria.
COBISS.SI-ID: 26953767