L2-4314 — Final report
1.
Modular serial multi-chamber flow-through electroporation device

Project partners from the Electrotehcnical faculty have resgistered a patent appliccation for the multi-chamber flow-through electroporation device developed during the project. This device was used experimentally for disruption of bacteria in water samples in a flow through format and confirms the proof of principle generated in this project: that Pulsed electric field can be used to eliminate bacteria from water samples.

F.32 International patent

COBISS.SI-ID: 10488404
2.
Dissemination of the project to society in the national Television.

Profesor Miklavcic presented and explained the fundaments of electroporation and its appliccation in different fields such as medicine, water cleaning, etc. in the divulgative program from the national Slovene television RTV Slovenia, Dobra ura z Boštjanom. The use of electroporation in the project was also described and apart from profesor Miklavcic, representatives from other project partners were also present, such as Nejc Rački and Maja Ravnikar from NIB and Marjeta Strazar from Domzale-Kamnik waste water treatment plant.

F.35 Other

COBISS.SI-ID: 10541652
3.
Proceedings of the COST TD1104 electroporation-based technologies and treatments

One of the lab works was made available to the students attending the international scientific workshop and postgraduate course Electroporation based technologies and treatments COST TD1104. The course was dedicated to inactivation of bacteria by irreversible electroporation. The protocol is based on the results of this project.

B.01 Organiser of a scientific meeting

COBISS.SI-ID: 269548544
4.
Dissemination of the results from the monitoring of seasonal changes in bacterial and viral population in Domžale-Kamnik waste water plant effluent

Changes of bacterial and viral populations were monitored monthly for the duration of one year in the effluent of DomžaleKamnik waste water treatment plant (WWTP). The composition of entire bacterial population was determined by denaturing high performance liquid chromatography (DHPLC) of amplified 16S rDNA gene. Additionally, bacteria from genus Clostridium were cultivated and strains identified as C. difficile werefurther typed into ribotypes. Viral populations of rotaviruses, astroviruses, noroviruses GG I and II, sapoviruses and hepatitis A virus were analysed after concentration of 5l volumes with CIMQA 8ml tube columns (BIA separations) colums, followed by real time quantitative RTqPCR detection. Our results show that the overall bacterial population in WWTP effluent varies in time, but the bacterial and viral intestinal pathogens are present throughout the year.

F.35 Other

COBISS.SI-ID: 512356664
5.
CIM monoliths as a tool for removing viruses from water. Presentation of the results to the international scientific community

We have continuously presented the results obtained when applying CIM monoliths for the removal of viruses from a wastewater plant's effluent to the international environmental virology audience.

B.03 Paper at an international scientific conference

COBISS.SI-ID: 30436569