V4-1073 — Annual report 2011
1.
Development of methods for determination of drought tolerance as a tool in plant breeding process

Morphological, physiological and genetic parameters of different hop and common bean varieties were studied with the aim to develop a method for determination of drought tolerance that can be applied in breeding programs. Drought stress can be efficiently evaluated with non-specific methods, where more parameters are considered and thus a comprehensive picture of the physiological status of plants is obtained. TRAP test is used to assess the resistance to oxidative stress or to determine the overall antioxidant activity of the sample examined. It proved to be an effective method for determination of drought tolerance, which can be directly used as a selection criteria in the breeding process of new hop and common beans varieties.

F.02 Acquisition of new scientific knowledge

COBISS.SI-ID: 560268
2.
SEED Net regional collecting expedition and ex situ conservation of Trifolium pratense L., Festuca pratensis Huds., Dactylis glomerata L. and Medicago falcata L.

South East Europe is a region rich in biodiversity and significant parts of the region are covered by grassland, constituting a great potential for utilizing such biodiversity in forage plants breeding programs. In order to ensure a long-term conservation of its valuable plant genetic resources and promote for a sustainable utilisation a number of national institutions in the region established SEEDNet (South East European Development Network on Plant Genetic Resources) in 2004 in order to strengthen the national efforts. Over the period of three years (2007-2010), collecting expeditions of ten project partners were focused on four fodder crops species and were carried out in different floristic, environmental or pedological regions belonging mainly to permanent grassland habitats. The main objective was to enlarge the genetic basis of regional forage genetic resources collections with new germplasm of two grass (Festuca pratensis Huds., Dactilys glomerata L.) and two legume species (Trifolium pratense L., Medicago sativa ssp. falcata (L.) Arcang.) defined as priority species for the region. In total 896 accessions were collected, from those 272 of cock's foot, 226 of meadow fescue, 313 samples of red clover and 85 yellow alfalfa samples.

B.03 Paper at an international scientific conference

COBISS.SI-ID: 3573096
3.
Conservation and utilization of Slovene common bean (Phaseolus vulgaris L.) germplasm

Common bean cultivation has a long tradition in Slovenia, which has resulted in the development of numerous landraces that are still grown today in addition to newly established cultivars. The Genebank of the Agricultural Institute of Slovenia (AIS) holds an ex situ collection of 1116 Phaseolus accessions (1058 P. vulgaris L., 58 P. coccineus L.); the majority (1055 accessions) are of Slovene origin. The results of a comprehensive study using molecular (AFLP, SSR, RAPD), biochemical (seed proteins) and morphological markers for evaluation of the genetic variation and relationships among and within autochthonous accessions were presented. In addition, the studies of drought stress using different approaches were presented as well.

B.03 Paper at an international scientific conference

COBISS.SI-ID: 3574632