Large quantities of antimicrobial peptides are required for investigations and clinical trials, therefore suitable production method is necessary. Production of recombinant antimicrobial peptides in prokaryotic systems is potentially limited due to their toxicity to host cells and susceptibility to proteolytic degradation, which can be avoided using fusion protein approach. Chemical reagents for cleaving peptide bond between antimicrobial peptides and fusion proteins such as diluted acid and cyanogen bromide are selective and provide large antimicrobial peptides in short time.
COBISS.SI-ID: 4469018