A set of pairs of parallel coiled-coil-forming peptides was designed and tested. The design based on maximizing the difference in stability between the desired pairs and the most stable unwanted pairs. We used N-terminal helix-initiator residues, favorable combinations of electrostatic and hydrophobic interactions, and a negative design based on burial of Asn residues. The 36 pair combinations of the eight peptides were analysed by CD. Each peptide formed a high level of alpha-helical structure exclusively in combination with its designed partner confirming the orthogonality of peptide pairs.
COBISS.SI-ID: 4534810
The invention describes a preparation of self-assembling nanostructures and a process for preparation of self-assembling nanostructures from polypeptides which are composed of at least three segments forming a coiled helix, which are assembling themselves into one sole polypeptide chain in a defined sequence which enables the composing into defined nano-cages or nano-nets. Nanostructures can be assembled from one sole polypeptide chain, yet which is composed of three, four or six segments forming coiled helices and are stacked into nano-cages, nano-nets or nano- tubes.
COBISS.SI-ID: 4381210
The invention represents a polypeptide material with adaptable pore properties, prepared by two- or three-dimensional combining of fusion proteins composed of at least two protein domains, where at least one domain is forming a coiled helix and at least one domain is a protein oligomerization domain. The invention relates to a polypeptide material which can be used in applications such as chemical catalysis and separation of molecules on the basis of their properties.
COBISS.SI-ID: 4382234