1.

Self-assembling polypeptide polyhedra

The invention relates to polypeptides capable of forming polyhedra by self-assembly. The polypeptide is composed of at least 12 coiled-coil-forming segments connected via linker segments, wherein each of the edges of the polyhedron is constructed of a pair of said coiledcoilforming segments. We described the design and process for preparation of self-assembling tetrahedron, the simplest polyhedron. Polypeptide chain comprising 12 coiled-coil-forming segments in a defined order was succesfully assembled into tetrahedron. The nanostructure was characterized using different methods.

F.33 Slovenian patent

COBISS.SI-ID: 35592197

2.

Design self-assembling polypeptide tetrahedron

We designed the tetrahedron that self-assembles from a single polypeptide chain comprising 12 concatenated coiled-coil-forming peptide segments separated by flexible peptide hinges. Self-assembling of the recombinat polypeptide is defined by the precise order of coiled-coil-forming segments and their pairwise interactions that form 6 rigid edges, each measuring approx. 5 nm. We characterized the obtained nanostructure using different methods, transmission electron microscopy, atomic force microscopy, dynamic light scattering. Split fluorescent protein attached to the termini of the polypeptide is reconstituted only by the correctly formed tetrahedron, while polypeptides with a deleted or scrambled segment order fail to self-assemble correctly.

B.04 Guest lecture

COBISS.SI-ID: 4911642

3.

Self-assembly of polypeptides into nanoscale structures

At the Conference of the Slovenian Biochemical Society in Maribor, we demonstrated the principle of self-assembling polypeptide nanostructure design. We presented the tetrahedron that self-assembles from a single polypeptide chain comprising 12 concatenated coiled-coil-forming peptide segments separated by flexible peptide hinges. Self-assembling of the recombinat polypeptide is defined by the precise order of coiled-coil-forming segments and their pairwise interactions that form 6 rigid edges, each measuring approx. 5 nm. We characterized the obtained nanostructure using different methods, transmission electron microscopy, atomic force microscopy, dynamic light scattering.

B.04 Guest lecture

COBISS.SI-ID: 4827162

4.

Cooperation in the editorial board on the scientific conference with international participation

As a member of editorial board Helena Gradišar cooperated at the 9th Congress of the Slovenian Biochemical Society

C.06 Editorial board membership

COBISS.SI-ID: 258116096

5.

Coliled-coil based designed polyhedra

At the Bristol University, Roman Jerala had lecture on the topic of the principle of self-assembling polypeptide nanostructure design. He presented the design of tetrahedron that self-assembles from a single polypeptide chain comprising 12 concatenated coiled-coil-forming peptide segments separated by flexible peptide hinges. Self-assembling of the recombinat polypeptide is defined by the precise order of coiled-coil-forming segments and their pairwise interactions that form 6 rigid edges, each measuring approx. 5 nm. We characterized the obtained nanostructure using different methods, transmission electron microscopy, atomic force microscopy, dynamic light scattering. Split fluorescent protein attached to the termini of the polypeptide is reconstituted only by the correctly formed tetrahedron, while polypeptides with a deleted or scrambled segment order fail to self-assemble correctly.

B.04 Guest lecture

COBISS.SI-ID: 4975898