J2-2040 — Annual report 2010
1.
Alcohol dehydrogenase in non-aqueous media using high-pressure technologies: reaction set-up and deactivation determination

Different reaction media were applied for biocatalysis, where alcohol dehydrogenase from Lactobacillus brevis, combined with its coenzyme, NADP, was used for the production of optically pure R-1-phenylethanol from acetophenone. Enzyme activity and stability was studied in these media.

COBISS.SI-ID: 14291222
2.
Surface functionalization of silica-coated magnetic nanoparticles for covalent attachment of cholesterol oxidase

The synthesis of bare maghemite nanoparticles was accomplished by thermal co-precipitation of iron ions in ammonia alkaline solution at harsh reaction conditions. Primary surface engineering of maghemite nanoparticles was performed by the proper deposition of silica onto nanoparticles surface under strictly regulated reaction conditions. The secondary surface functionalization was achieved by coating the particles with organosilane, followed by glutaraldehyde activation. Covalent immobilization of cholesterol oxidase was attempted afterwards.

COBISS.SI-ID: 13418262