P4-0116 — Annual report 2009
1.
Differences in the activity and bacterial community structure of drained grassland and forest peat soils

This is the first comparative study of microbial activity and bacterial community structure in drained fen and bog soils of the Ljubljana Marsh, Slovenia, different in pH and soil organic matter content. The substrate induced respiration (SIR) was higher in the bog soil, but lower when CO2 production was expressed per volume of soil. Bacterial communities were assessed by (T-RFLP) profiling of 16S rRNA genes and indicated differences between the two soils, which were rich with Acidobacteria but contained a distinct set of phylogenetic subgroups of this important group of bacteria.

COBISS.SI-ID: 3658872
2.
In vitro antimicrobial and antioxidant activity of commercial rosemary extract formulations

As it is obvious from the title of the article, we published comparative analysis of the results of antimicrobial and antioxidant activity of commercial phenolic extracts of rosemary (Rosmarinus officinalis), used as food additives. We analysed the results collected by different methods of testing antimicrobial and antioxidant activity and found joint mechanisms involved in both types of activity. Beside this, tha analysis was a basis for further selection of testing methods, which were later applied for testing new potential antimicrobial and antioxidant additives for food industry.

COBISS.SI-ID: 3604088
3.
Regulatory elements in tetracycline-encoding gene clusters: the otcG gene positively regulates the production of oxytetracycline in S. rimosus

A detailed »in silico« analysis of regulatory elements in the oxytetracycline, chlortetracycline and chelocardin gene clusters was performed. Within chlortetracycline gene cluster, three regulatory genes were identified, within oxytetracycline only two, and within chelocardin only one transcriptional regulator was identified. In the oxytetracycline gene cluster, a regulatory element that is not typical for aromatic polyketides was identified. The otcG gene was cloned and its putative »conditionally-positive« role was experimentally demonstrated.

COBISS.SI-ID: 3608696
4.
Saccharomyces cerevisiae : the effect of different forms and concentrations of iodine on uptake and yeast growth

In this publication the effect of different forms of iodine compounds on accumulation and growth of yeast Saccharomyces cerevisiae was presented. We investigated the influence of different sources of iodine, on its uptake in and growth of the model yeast. We also investigated the specificity of iodine uptake in the presence of selenate, zinc and chromate in the growth medium. We found that KIO3 inhibited the growth of the yeast and the chromate had the strongest influence on reduction of iodine uptake.

COBISS.SI-ID: 22024743
5.
The effect of ATP sulphurylase on the prooxidant properties of selenate in yeast Schizosaccharomyces pombe

Selenium is an essential microelement in human and animal nutrition and prooxidant effect of inorganic selenium sources has been found as a great disadvantage. In this study the effect of the ATP sulphurylase on the prooxidant properties and toxicity of selenate in the fission yeast model organism Schizosaccharomyces pombe has been studied. We found out that active ATP sulphurylase is the key enzyme for the prooxidant properties of selenate and the main reason for selenate toxicity and ROS formation during the selenate reduction.

COBISS.SI-ID: 3584120