P4-0097 — Annual report 2010
1.
Molecular profiling and identification of methanogenic archaeal species from rabbit caecum

A unique 16S rDNA band common to all rabbit caecum samples was observed during DGGE profiling of methanogenic archaea from rumen fluid, rabbit caecum and pig feces. Ssu rRNA sequences of methanogenic archaea were subsequently retrieved from the constructed rabbit caecum 16S rDNA gene library. Results indicated that rabbit caecum is inhabited by members of the genus Methanobrevibacter and is possibly one-species dominated. This species is novel and belongs to a distinct phylogenetic group containing strains isolated from animal and human feces

COBISS.SI-ID: 2744712
2.
Effects of two probiotic additives containing Bacillus spores on carcass characteristics, blood lipids and cecal volatile fatty acids in chickens

In this work the effects of two commercial probiotic feed additives containing Bacillus subtilis and B. licheniformis spores (A) or B. cereus var. toyoi spores (B), on carcass and meat characteristics, serum lipids and cecal volatile fatty acids were evaluated on 120 meat type chickens. Beside some positive effects on the weight, yield and cecal fermentation, negative effects were observed as well such as unfavourable changes of animal’s blood serum cholesterol profile and increase of abdominal fat and meat conductivity.

COBISS.SI-ID: 2755464
3.
Quantification of Lactobacillus gasseri, Enterococcus faecium and Bifidobacterium infantis in a probiotic OTC drug by Real-time PCR

Although probiotics are widely used in medicinal products, there are no generally accepted methods for their quantification. Molecular methods for probiotics quantification have been extensively developed, however they are still not widely used in routine laboratories. In this work we introduced selective quantification of Bifidobacterium infantis, Lactobacillus gasseri and Enterococcus faecium in OTC probiotic drug by real-time PCR. The described methodology could complement the presently used culture-based quantification of bacteria and contribute to better control of probiotic products

COBISS.SI-ID: 2476680
4.
The effect of vitamin E and plant extract mixture on oxidative stress induced by high PUFA load in young pigs

The objective of our study was to determine the antioxidative potential of a plant extract (PE) mixture composed of carvacrol, capsicum oleoresin and cinnamaldehyde against high n-3 polyunsaturated fatty acid (PUFA)-induced oxidative stress in young pigs. The results show that PE and Vit E supplemented to pigs in concentrations of 271.2 mg/kg and 90.4 mg/kg, respectively, can effectively protect pig’s blood lymphocytes against oxidative DNA damage, thus suggesting their potentially beneficial effects on the immune system under dietary-induced oxidative stress

COBISS.SI-ID: 2543240
5.
DNA encoding for an efficient 'Omics processing

This paper proposes novel coding approaches, for both the dissemination and processing of DNA sequences, where the speed of the processing is shown to be boosted by exploring more than the normally utilized eight bits for encoding a single nucleotide. Further gains are achieved by encoding the nucleotides together with their trailing alignment information as a single 64-bit data structure. The paper also proposes a slight modification to the established FASTA scheme in order to improve on its representation of alignment information

COBISS.SI-ID: 2625416