Using 2D electrophoresis, we compared proteomic profile of biceps femoris muscle (insoluble fraction) according to genotype (PRKAG3Ile199Val and CASTLys249Arg/Ser638Arg), as well as salt content and pastiness. The gene PRKAG3 affected mainly metabolic enzymes. The impact of the CAST gene was smaller compared to PRKAG3, however, we could identify a link with fragments of actin. Salt content and pastiness were associated with metabolic and myofibrillar proteins, which can be ascribed to differences in proteolysis. Significant differences between proteomic profile based on pastiness and salt content indicates the possibility to search for potential markers of quality at the proteomic level.
COBISS.SI-ID: 3517032
About 250 bumblebee species in 15 subgenera are known in the world. Identificantion of some species is difficult due to small morphological differences. In this study wing venation pattern was analyzed and obtained characters were used for species identification. 469 bumblebees from 121 localities in Slovenia and 61 imported individuals were included in the analyses. The co-ordinates of 19 vein crosses on the forewings were measured and used in the calculation of 37 wing characters. Based on discriminate method more than 97 % of all analysed bumblebees were assigned to the correct species. The most informative characters were angles J16, A4 and discoidal shift. Wing venation pattern also differed between autochtomous and imported Bombus terrestris individuals.
COBISS.SI-ID: 3630696
The aim of this work was to determine the influence of different storage temperatures and tuber maturity on development of tuber necrosis caused by Potato virus Y NTN . Infected tubers of the cultivar Igor of two different maturity groups and non-infected tubers were randomly distributed into 12 storage regimes. Control treatments were stored at constant temperatures of 4, 13 and 24°C. Tubers in the other nine treatments were stored at 4°C at the beginning of experiment and transferred to 24°C after 1 to 29 weeks after harvest. High storage temperatures enhanced the development of tuber necrosis. Prolongation of low temperature storage delayed the development of necrosis and reduced the number of tubers with necrosis. When tubers were stored at low temperature for 21 weeks or more, no necrosis developed after transfer to high temperature. We could not confirm the effect of maturity on development of necrosis caused by PVYNTN.
COBISS.SI-ID: 3597928