P4-0072 — Annual report 2009
1.
Characterisation of the root-knot nematode, Meloidogyne ethiopica Whitehead, 1968, from Slovenia.

A Meloidogyne ethiopica population was isolated from heavily infested tomato roots in a greenhouse in Slovenia. This was the first finding of M. ethiopica in Europe. The species was identified by morphometrical analyses and confirmed by isozyme electrophoresis. Identification of the species was completed by comparison of rDNA sequences with other Meloidogyne species, where our sequence clustered with Brazil population of M. ethiopica. Twenty-two new host plants for M. ethiopica were established in our bio-tests.

COBISS.SI-ID: 3200360
2.
Biological, serological and molecular characterisation of Raspberry bushy dwarf virus and its detection in the nematode L. juvenilis

Raspberry bushy dwarf virus (RBDV) was found in grapevine in Slovenia in 2003. This was the first reported natural infection of non-Rubus host with RBDV. The grapevine isolate was characterized serologically, its experimental host range was investigated and most of RNA 2 was sequenced. The grapevine isolate is clearely different from slovenian red raspberry isolate. Additionaly, the presence of RBDV in nematodes Longidorus juvenilis was detected what indicates a possible role of nematodes in RBDV transmission. The possibility of nematode transmission of RBSV was never reported before.

COBISS.SI-ID: 2739304
3.
A quantitative technique for determining proteases and their substrate specificity and pH optima in crude enzyme extracts.

A zymography technique, applied to aminopeptidases and peptidases in crude bean leaf extracts, based on native polyacrylamide gel electrophoresis (PAGE) has been devised, which enables the substrate specificities, content and pH profiles of proteolytic enzymes to be determined in an unfractionated tissue extract. One enzyme active against Ala–AMC (7-amino-4-methylcoumarin), one enzyme active against Z-Arg–AMC, several enzymes active against Leu–AMC, and (for the first time in plants) several enzymes active against Phe–AMC were identified.

COBISS.SI-ID: 3576952
4.
Biodiversity of agriculturally relevant and opportunistic human pathogenic Fusarium species

The Fusarium dimerum species group represents a monophyletic subclade among other Fusarium groups, all accommodating important plant pathogens and some mycotoxin producers and opportunistic human pathogens. Three species are newly described. Opportunistic human pathogenicity is largely confined to the species clade of F. dimerum. Fusarium domesticum, a colonizer of cheese but, most likely, neither mycotoxigenic nor human or plant pathogenic, is the closest phylogenetic sister of the F. dimerum species group. DNA barcodes allowing precise species identifications are provided.

COBISS.SI-ID: 2915944
5.
Effect of I199V polymorphism on PRKAG3 gene on carcass and meat quality traits in Slovenian commercial pigs

The results of the study demonstrate a beneficial effect of allele I on meat quality, but also negative one on leanness. The present study provides data for the genotype I/I, which are lacking due to the low incidence of allele I in the majority of modern breeds.

COBISS.SI-ID: 3033448