P4-0013 — Annual report 2009
1.
European elderberry (Sambucus nigra L.) rich in sugars, organic acids, anthocyanins and selected polyphenols

Elderberry is a known medicinal plant, in recent years it has been cultivated as fruit crop, valuable source of anthocyanins – red pigments, mainly used for processing. With HPLC/MS we detected anthocyanins cyanidin-3-sambubioside-5-glucoside, cyanidin-3,5-diglucoside, cyanidin-3-sambubioside, cyanidin-3-glucoside and cyanidin-3-rutinoside. The highest total anthocyanin content was 1265 mg/100 g FW fruit. From the quercetin glucosides group we identified quercetin-3-rutinoside and quercetin-3-glucoside (in total 73,4 mg/100 g FW). Elderberry fruit is also rich in organic acids (6,38 g/kg FW).

COBISS.SI-ID: 5697913
2.
Anthocyanins and fruit colour in plums (Prunus domestica L.) during ripening.

During 4 plum cultivars ripening we monitored color (colorimetrically) and anthocyanin content (HPLC/MS) in fruit skin. Correlation between color parameters L*, a* and b* and anthocyanin content was different for cultivars and ripening stage. Major anthocyanin was cyanidin-3-rutinoside (4,1 – 23,4 mg/100 g FW, from 52,6% to 73,0% total anthocyanins - TA), followed by peonidin-3-rutinoside (from 6,5% to 37,9% TA), cyanidin-3-glucoside (from 1,8% to 18,4% TA), cyanidin-3-xyloside (from 4,7% to 7,8% TA) and peonidin-3-glucoside (from 0.0 % to 0,4% TA).

COBISS.SI-ID: 5838201
3.
Nontarget effect of entomopathogenic nematodes on larvae of twospotted lady beetle (Coccinellidae) and lacewing (Chrysopidae) in laboratory conditions

We monitored the impact of entomopathogenic nematodae (Steinernema feltiae, Steinernema carpocapsae, Heterorhabditis bacteriophora) on larvae of Adalia bipunctata and Chrysoperla carnea. Experiment was conducted in lab at 3 temperatures (15, 20 and 25 o C) and 3 concentrations of nematodae suspensions. Larvae of Adalia bipunctata were more prone to attack of nematodae than larvae of Chrysoperla carnea. Lower mortality of all larvae after 4 days was detected at 15 o C, however no statistically significant differences were observed at other temperatures and concentrations of nematodae.

COBISS.SI-ID: 6045305
4.
Changes in the phenolic concentration during flower development of rose 'KORcrisett'.

In developing rose flower ‘KORcrisett’ concentration of anthocyanins, quercetins, catechin and phenolic acids by the use of HPLC/MS was monitored. Development of color was measured colorimetrically. Buds and closed rose flowers contained up to threefold levels of pelargonidin-3,5-diglucoside and fourfold levels of cyanidin-3,5-diglucoside compared to senescent flower. Additionally, buds contained higher levels of quercetins, catechin and phenolic acids. A tight correlation was calculated between colorimetrically obtained parameters a*, b*, a*/b*, h°, L* and major/total anthocyanins.

COBISS.SI-ID: 6175353
5.
Accumulation of phenolic compounds in apple in response to infection by the scab pathogen, Venturia inaequalis.

Content of phenolics in healthy and apple scab infected leaf and fruit tissue was monitored by HPLC/MS. Leaf samples were collected from May to September, and fruit samples at technological maturity. Infection with fungus increased the content of phenolics mostly in the border tissue between the infected and healthy tissue. Tissue infected with fungus Venturia inaequalis contained as much as 7,6 times more hydroxycinnamic acids, up to 2,6 more flavan-3-ols and up to 2,9 more flavanols. Total phenolic content was up to 2,4 times higher in infected tissue compared to healthy leaves and fruit.

COBISS.SI-ID: 6083961