The effects of an early local adjunct treatment on the course of pneumonia and inflammatory/cytokine response was studied in mice intratracheally inoculated with live Porphyromonas gingivalis and treated with either clindamycin (C), dexamethasone (D), C+D combination, or were not treated (Pg). Levels of tumor necrosis factor (TNF)-α, soluble TNF-α receptors (sTNFR1 and sTNFR2), interleukin (IL)-1β, and IL-6 in the serum and lung-homogenate supernatant were determined. Lung samples were histopathologically assessed and all findings compared to sham-inoculated mice (PBS). Clindamycin-treated mice developed only mild bronchopneumonia that resolved fast (72 hours) with an early (6–24 hours) normalization of local and systemic cytokine levels. Similar course of pneumonia and cytokine level changes were observed in mice treated with C+D, but later. Clindamycin or a clindamycin-dexamethasone combination treatment significantly improves the course of P. gingivalis-aspiration pneumonia, but more so if clindamycin alone is used.
COBISS.SI-ID: 3475066
Twenty-four BALB/c mice were orally inoculated with 10(8) CFU Escherichia coli ATCC 25922 and euthanized 2.5, 7, 13 and 25h post-inoculation. The levels of organ nitric oxide (NO) in lungs, liver, kidneys and brain, and plasma endotoxin, TNF-alpha and nitrite/nitrate (NO(x)) were compared to those found in sham-inoculated mice, to evaluate systemic host-response to a low-level oral exposure to Gram-negative bacteria. Organ NO and plasma TNF-alpha levels were higher in E. coli-inoculated animals, but no differences were detected in plasma endotoxin levels and NO(x) for any of the animal groups. Single oral gavage with live E. coli stimulates an early systemic immune response in clinically healthy mice as evidenced by increased plasma TNF-alpha and organ NO levels.response in clinically healthy mice as evidenced by increased plasma TNF-alpha and organ NO levels, but bacteremia and endotoxemia are not related to this inflammatory response.
COBISS.SI-ID: 3335546
We evaluated and identified morphological characteristics of Class III children in deciduous dentition using a non-invasive 3D laser surface scanning method, for more effective, improved, non-invasive diagnosis and enhanced treatment planning. A 3D laser imaging method could in the future become an important diagnostic tool in small children. The most important clinical advantage of this study is the non-invasiveness of the diagnostic method.
COBISS.SI-ID: 29626585
The paper describes free temporal fascia transfer for the replacement of missing mucosa of the floor of the mouth. Surgery was performed for the first time in Slovenia and represents a contribution to orofacial reconstruction of cancer patients.
COBISS.SI-ID: 28187609
The aims of the study were to identify craniofacial characteristics in patients with the rough hypoplastic amelogenesis imperfecta (AI) phenotype andto evaluate whether craniofacial variables are related to a mutation in either of the two genes associated with AI, enamelin (ENAM) and amelogenin (AMGX). Eight children (five males and three females) with rough hypoplastic AI phenotype, aged 6.5-15 years, from three families and their parents (three males and three females) were examined clinically, radiographically, and genetically. Seventeen variables were measured on lateral cephalometric radiographs in AI affected (n = 11) and AI unaffected (n = 3) members. Craniofacial measurements were statistically analysed using a Studentćs t-test. In all 14 individuals, mutation analysis of the ENAM and AMGX genes was performed by direct sequencing of the coding region. All AI affected patients had hypoplastic enamel with a rough surface and malocclusions. In thevertical plane, all AI children presented an anterior and/or posterior openbite (OB). Craniofacial analysis confirmed increased vertical relationships, with increased vertical jaw relationships and higher values forgonial angle. In two AI affected families, A and B, the same heterozygous ENAM g.8344delG mutation was confirmed, while in the third family, neither ENAM nor AMGX mutation was found. All patients with rough hypoplastic AI had amoderate to severe malocclusion with increased vertical dimensions regardlessof the presence or absence of the ENAM g.8344delG mutation. As an OBrequires appropriate timing of therapy, it is important to diagnose these patients as early as possible.
COBISS.SI-ID: 27607513