A new biophysical model of fusion pore stability is shown, based on the accumulation of anisotropic membrane constituents in highly curved membrane areas. This model predicts relatively high density of anisotropic constituents in relatively small membrane bound vesicles. These vesicles therefore exhibit stable fusion pores with relatively narrow fusion pore diameters. Experimental data supports this hypothesis. Therefore this study represents the first case, where the size of vesicles is placed into a a physiological context, and that this determins the size of the stabe fusion pore diameter.
COBISS.SI-ID: 26735321
This review was produced within the frame of the scientific committee at European Medicine's Agency and deals with key scientific and regulatroy challenges related to the development of advanced medical products.
COBISS.SI-ID: 26685401
This review paper describes initially the key criteria to list signal molecules into the category of tranmitters in glial cells. Then there are mored detailed descriptions of the properties of specific molecules: glutamate, ATP, D-serine, peptides.
COBISS.SI-ID: 26895833
In this paper the mobility of three classes of vesicles in astrocytes was addressed, in particular their mobility following the application of agents which increase cytosolic activity of calcium ions. The results have shown that in the absence of intermediary filaments stimulation-induced mobility change was no longer present, indicating an essential role of these cytoskeletal elements in regulating vesicle traffic in astrocytes.
COBISS.SI-ID: 27046873
In mammals taste buds consist of three cell types. The paper describes experiments of taste cells, specifically labelled fluorescently in different transgenic mise. We have used membrane capacitance measurements to monitor membrane surface area fluctuations which are due to exo- and endocytosis. The results in this paper show that only in type III taste cells membrane depolarizations evoke a significant increase in membrane capacitance, which likely represents Ca2+-dependent regulated exocytosis, which may play a role in the taste transduction.
COBISS.SI-ID: 27569369