1.

Characterisation of the capillary network in skeletal muscles from 3D data;

This review article presents methodology for capillary analysis from 3Ddata. Two methods were implied: i) counting transsections of capillaries with virtual stereological grids, superimposed into the stack of optical images ii) tracing capillaries in virtual 3D space. Attention is paid to preparation of muscle samples for the analysis and to possible artifacts. The methods are illustrated with the results of anatomical remodelling of the capillary network in muscle of rats, undergone acute denrevation and early reinnervation.

COBISS.SI-ID: 28184537

2.

A novel method for evaluation of capillarity in human skeletal muscles from confocal 3D images;

In this paper we present for the first time i) immunofluorescent methods for the demonstration of capillaries in 100µm thick sections of human muscles and ii) methods for automatic segmentation of capillaries from thick tissue sections, followed by manual corrections applying haptic device. Special attention is paid to the sample preparation. We introduce corrections for tissue shrinkage. The methods are illustrated by the data on the capillary length in differently defined reference space as well as by data on the orientation of capillaries in three human muscles with different function.

COBISS.SI-ID: 27856345

3.

Acetylcholinesterase is involved in apoptosis in the precursors of human muscle regeneration;

In addition to the established cholinergic function of AChE, there is increasing evidence for its non-cholinergic functions. Due to reports demonstrating AChE involvement in apoptosis and due to its early expression at the myoblast stage, we hypothesized that at this stage AChE has proapoptotic role. By finding decreased apoptosis in siRNA AChE silenced myoblasts and increased AChE expression in staurosporine-treated myoblasts we confirmed this hypothesis. AChE splice variants were differently affected by staurosporine-induced apoptosis with the highest increase of R-AChE variant.

COBISS.SI-ID: 27660761

4.

HIF-1[alpha] response to hypoxia is functionally separated from the glucocorticoid stress response in the in vitro regenerating human skeletal muscle

The aim of this study was to investigate whether the cellular response to hypoxia, organized by hypoxia-inducible factor-1? (HIF-1?) interacts with the stress response, organized by glucocorticoids. Expression of HIF-1a had no influence on the expression of the glucocorticoid response markers in the glucocorticoid-treated myoblasts which suggests mutual independence of both systems at the organization of the response to hypoxia. This finding is important since myoblast proliferation is a part of muscle regeneration after injury, where hypoxia and stress response are both present.

COBISS.SI-ID: 27660761

5.

Near-infrared spectroscopy during stagnant ischemia estimates central venous oxygen saturation and mixed venous oxygen saturation discrepancy...;

In this report we demonstrated that non-invasive near-infrared spectroscopy during stagnant ischemia can be used for the estimation of the discrepancies in central venous oxygen saturation and mixed venous oxygen saturation in patients with severe left heart failure. This is not possible in patients with accompannying sepsis/septic shock. The results of this investigation will change our approach how to target therapy and follow changes during diagnostics and therapy in critically ill.

COBISS.SI-ID: 27400665