A new electroporation monitoring method that allows direct measurement of the electric field strength during the treatment was introduced. The method is able to sense an insufficient electric field distribution before the end of the treatment which is essential for improving the electroporation treatment. As measurement of the electric field inside the tissue is impossible we established indirect approach using magnetic resonance technique. Electric field is obtained by measured conductivity of the tissue and current density established during the application of pulses.
COBISS.SI-ID: 8693332
The use of electric pulses to increase cell membrane permeability – electroporation – has also been used on skin for (a) enhanced transdermal molecular delivery or (b) the delivery of drugs or DNA into viable skin cells. Based on finite element numerical method, we theoretically described skin electropermeabilization and the amount of heating in and around an electrically created pore in the stratum corneum (SC). With the model, we address both, electrical as well as thermal effects on skin tissue.
COBISS.SI-ID: 8282708
In this paper a novel parameter optimization approach for cell detection tool and the cell counting procedure were presented. The automated cell counting procedure is based on novel artificial neural network optimization of Image-based Tool for Counting Nuclei algorithm parameters to fit the training image set based on counts from an expert. Comparing the results of the automated cell counting to user manual counting a 90 % average agreement was achieved which is reasonably good especially taking into account inter-person error which can be up to 10%. The electroporation efficiency determined by manual cell counting was virtually the same as the one obtained by our automated procedure.
COBISS.SI-ID: 8213332