An original real-time PCR assay based on FRET hybridization probe technology, allowing detection of HPV-6 and HPV-11, reliable differentiation of HPV-6 and HPV-11, as well as prototypic and non-prototypic HPV-6 genomic variants, in a single PCR reaction, was described and published in a well-known journal in the field of contemporary virological methods. The assay proved to be highly sensitive, specific and reproducible.
COBISS.SI-ID: 25489881
In the study we determined distribution of HPV genotypes in a representative cohort of women with cervical cancer (CC) in Slovenia to contribute to the lacking data on HPV in CC and to assess the potential benefit of future prophylactic HPV vaccination. HPV genotypes found decreasingly were: HPV 16, 18, 33, 31, 51, 58, 59, 35, 52, 73 and 82. Our study showed that prophylactic HPV vaccination with available vaccines could prevent up to 77.1% of CC in Slovenia, which is caused by HPV 16 or 18.
COBISS.SI-ID: 25690841
In this study we compared detection of HPV genotypes in 40 paired cervical scrape samples and tumor tissue samples of 40 patients with cervical cancer (CC). Overall, 39/40 (97.5%) of paired CC samples were HPV positive; overall agreement between paired samples was 98.75%. Cervical scrape samples are equally useful for HPV genotype determination as tumor tissue samples in patients with CC, and can be used as accurate clinical samples for detection of HPV causing CC or for epidemiological studies.
COBISS.SI-ID: 26239449
Genomic diversity of HPV-6 was established by sequencing 77 HPV-6 isolates from 45 and 32 patients with genital warts (GW) and laryngeal papillomas (LP). By analyzing L1-LCR-E6-E2-E5 nucleotide data of individual isolate, a total of 36 genomic variants were identified, of which six, one and 29 corresponded to HPV-6b, -6a, and -6vc genetic lineages. Several potentially important mutations were identified. Non-prototypic HPV-6vc variants were found in the majority of GW and LP included in study.
COBISS.SI-ID: 25836761
The Hybrid Capture 2 low-risk (hc2-LR) is a molecular test for detection of clinically most important LR-HPV genotypes 6, 11, 42, 43, 44. To determine specificity of hc2-LR, a genotyping study of 285 hc2-LR positive samples was done and showed cross-reactivity (CR) with several untargeted HPVs. CR was often clinically beneficial due to detection of untargeted LR genotypes. 8.4% of positive results, usually weak, were due to CR with high-risk genotypes. Introduction of gray zone is recommended.
COBISS.SI-ID: 25779929