At the International symposium on double-stranded RNA viruses a study, comparing different concentrating methods was presented. Viruses were concentrated using various positively charged filters, eluted with two different elution buffers. There were significant variations of efficiency between different filters and elution buffers. According to the data obtained the optimal technique was used for additional testing.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 3088250An efficient alternative for the current concentration technique was presented at the COST929 symposium. Our presentation was an important contribution in Slovenian science promotion abroad. The new chromatographic method was tested comparable to the commonly used ultrafiltration technique. Comparing to the ultrafiltration method the virus concentration factor was 3-4-times improved when using our new developed method. The disadvantage of our new method is a need for virus-specific conditions.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 25081817Molecular epidemiology data of group A rotaviruses detected in 5 years old children with rotavirus gastroenteritis in Slovenia was presented. The study was part of the EuroRotaNet project. Rotavirus strains with common rotavirus G and P genotypes were detected as the most prevalent strains. However, a high percentage of unusual genotypes detected mostly in bovine rotavirus strains were characterized. The animal source of these strains was confirmed after a detailed molecular analysis.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 26802649At the European Rotavirus Biology Meeting an update of the Rotavirus Classification Working Group was presented. RCWG is a network of worldwide experts in the field of rotavirus molecular research. The aim of this network is to collect and suggest new changes in rotavirus classification in close collaboration with the International Committee on Taxonomy of Viruses (ICTV). Dr. Andrej Steyer is a member of the RCWG since its establishment.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 26803161In this presentation at the European Rotavirus Biology Meeting the zoonotic transmission of bovine rotavirus strains to human was presented and four genome segments coding for important proteins involved in viral pathogenesis were analyzed (VP4-outer capsid protein, VP7- outer capsid glycoprotein, VP6-inner capsid protein, NSP4-enterotoxin). As reassortment of rotavirus genome is possible and a new rotavirus vaccine was recently introduced it is important in the future to follow the introduction of new, unusual rotavirus strains into human population.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 26803417