Cross-contamination of the cell lines, which are used in research and in biotechnological processes, represents a huge problem and for this reason, the cell lines must be well defined. The methods, which must be used in the research work as well as in industry to assure a quality measures as well as the accurate and reproducible results, are proposed and described in our paper. Industrial partners have expressed their intent to implement our technology for the characterisation of the cell lines, which are used in their production.
COBISS.SI-ID: 24001241
Based on the synthetic biology cloning vector pSB1AC3 we prepared a prokaryotic expression vector pMD204 which is adapted for expression of fusion proteins in Escherichia coli. The outline of the vector is such that stepwise insertion of L- and H-chain variable domains and of the linker peptide coding regions will be possible.
COBISS.SI-ID: 30190085