Tumor vasculature is due to the specific proterties that makes is different from normal vasculature, an attractive target for treatment. In the study, we demonstrated that electroporation reduces tumor blood flow. In addition, we showed that electrochemotherapy, besides affecting tumor blood flow, act cytotoxic on tumor endothelial cells, contibuting to overall antitumor effectiveness of this therapy.
COBISS.SI-ID: 21432615
The results of our study showed that electroporation is feasible and effective method for delivering of plasmid DNA expressing shRNA into tumor cells in vitro, which was shown through efficient silencing of targeted reporter gene for GFP. Imaging and measurements of GFP expression levels over time in vitro showed that electroporation in combination with plasmid DNA as gene delivery vector can induce prolonged gene silencing effect in rapidly dividing LPBGFP cells.
COBISS.SI-ID: 24489689