L4-9442 — Final report
1.
RADIŠEK, Sebastjan. Verticillium Wilt

The problems and knowledge of Verticillium wilt caused by Verticillium spp. is covered.

COBISS.SI-ID: 651127
2.
First viroid-free hop plants (Humulus lupulus L.) in Slovenia

The Slovenian Institute for Hop Research and Brewing has started the elimination of Hop latent viroid (HLVd) and obtained first viroid-free mother plants. Healthy planting material is a prerequisite to get high and quality yield. Meristems were excised from newly emerged sprouts of mother plants and growing them on appropriate growing media. In vitro regeneration varied between 18 and 60%, depending on variety. Viroid-free tested plants were transferres to in vivo conditions and are used as mother plants for further propagation of certified hop planting material.

COBISS.SI-ID: 715895
3.
Biological, serological and molecular characterisation of RBDV from grapevine and its detection in the nematode Longidorus juvenilis

RBDV was until recently, found only on Rubus species. In 2003 we reported a first finding of RBDV on grapevine as non-Rubus natural host. In this paper we report about about further biological, serological and molecular characterisation of grapevine isolates. They differ from Slovenian isolate of RBDV from raspberry. RBDV was detected also in the nematode Longidorus juvenilis. The results show a bigger diversity inside RBDV than previously thought. Finding of RBDV in L. juvenilis shows a possibility of virus transmission by nematodes which was not known before for this virus.

COBISS.SI-ID: 2739304
4.
RADIŠEK, Sebastjan, JAKŠE, Jernej, JAVORNIK, Branka, DE GRUYTER, J. First report of Phoma exigua as a pathogen of hop in Slovenia

In August 2005, necrotic lesions on cones and leaves were observed on hop in Slovenia. Microscopic examination revealed the presence of irregularly scattered globose pycnidia . The pathogen was identified as Phoma exigua Desm. The identification was confirmed by sequencing the ITS region. Pathogenicity tests were performed and the fungus was re-isolated from the lesions. To our knowledge, this is the first report of P. exigua causing severe damage to hop.

COBISS.SI-ID: 5000057
5.
VIRŠČEK MARN, Mojca, MAVRIČ, Irena, ZINDOVIĆ, Jelena. Discovery and characterisation of Plum pox virus isolates in Montenegro

In 2006 20 plum orchards in Montenegro were screened for the presence of Plum pox virus (PPV). Symptoms were found in 15 orchards. 19 samples were collected and 17 proved to be positive by DAS-ELISA. Of these 16 were selected for molecular identification. The results showed the presence of PPV-D type isolates in 4 samples. The remaining 12 samples were PPV-M positive by TAS-ELISA. Of these, seven proved to be of PPV-REC. Other five isolates were further analyzed and identified as the PPV-REC type. To our knowledge this was the first confirmation of PPV infection in Montenegro.

COBISS.SI-ID: 2555752