1.

Isolation of tomato pectin methylesterase and polygalacturonase on short monolithic columns

We developed new extraction, isolation procedure for the isolation of the enzymes pectin methylesterase (PME) and polygalacturonase (PG) from fresh tomato fruit. One of the main forms of tomato PME that is applicable to the food industry and one form of PG were isolated using short monolithic columns (CIM disks, Bia Separations). Times cited: 11

COBISS.SI-ID: 3561754

2.

New HPLC-UV and LC-MS methods for determination of triterpenoids in epicuticular wax of cabbage

Lupeol, together with ?-amyrin and ß-amyrin in smaller quantities, has been found for the first time in the epicuticular wax of white cabbage leaf surface extract. The combination of developed complementary chromatographic techniques, TLC on silica gel and HPLC on reversed-phase, with several detection techniques including APCI-MS-MS, was crucial for identification of these isomeric and can be applied to the analysis of other plant extracts.

COBISS.SI-ID: 3751962

3.

A new cutting edge HPLC method for the separation of shikonin and its derivatives

The development of a new isocratic HPLC method enables simultaneous separation and identification of nine structurally highly resembling shikonin derivatives (even positional and geometric isomers) for the first time: shikonin, acetylshikonin, propionylshikonin, isobutyrylshikonin, tigloylshikonin, 3,3-dimethylacrylshikonin, angelylshikonin, 2-methyl-n-butyrylshikonin and isovalerylshikonin. These biologically active compunds were identified for the first time in Echium italicum L. by this newly developed HPLC method, NMR and MS spectra.

COBISS.SI-ID: 4134682

4.

A new optimised TLC detection reagent for identification and quantification catechins in different plant materials

Catechins are important bioactive compounds in the plant kingdom. Consumption of food (green tea, red grapes, cocoa etc.) rich with catechins is associated by prevention of chronic diseases. Until now, for TLC detection of catechins aromatic aldehyde vanillin has mainly been used. The main drawback of vanillin reagent is rapid discoloration of chromatographic bands. Therefore, instead of vanillin we used 4-dimetylaminocinnamaldehyde. The reagent containing this aldehyde gives more stable bands also less aldehyde and acid are needed for the preparation of this reagent.

COBISS.SI-ID: 4134938

5.

Methacrylate-based short monolithic columns:enabling tools for rapid and efficient analyses of biomolecules and nanoparticles

The basic structural and chromatographic properties of short monolithic analytical columns were presented for the first time.

COBISS.SI-ID: 3469176