J4-9476 — Annual report 2008
1.
The efficiency of AFLP and SSR markers in genetic diversity estimation and gene pool classification of common bean (Phaseolus vulgaris L.)

The present work was conducted to evaluate AFLP (Amplified Fragment Length Polymorphism) and SSR (Simple Sequence Repeat) marker systems for their ability to detect genetic diversity within a set of 29 common bean accessions spanning both the Andean and Mesoamerican gene pools and to compare the efficiency of these two marker types in the classification of accessions according to the gene pools of origin. Results show that both marker systems are successful in evaluation of genetic diversity of common bean and its classification according to gene pool of origin.

COBISS.SI-ID: 5590905
2.
Identification of genes involved in the response of leaves of Phaseolus vulgaris to drought stress.

The identification of genes whose expression is altered under conditions of drought is an important first step towards understanding the response of this species. Changes in gene expression in their leaves at different levels of dehydration were identified by differential display reverse transcriptase PCR and confirmed by quantitative real-time PCR. The levels of 15 transcripts were changed significantly (p<0.05) in all cultivars under both growth conditions, 8 being increased and seven decreased. Five of the genes identified have not previously been reported as being influenced by drought.

COBISS.SI-ID: 2463336