A zymography technique, applied to aminopeptidases and peptidases in crude bean leaf extracts, based on native polyacrylamide gel electrophoresis (PAGE) has been devised, which enables the substrate specificities, content and pH profiles of proteolytic enzymes to be determined in an unfractionated tissue extract. One enzyme active against Ala–AMC (7-amino-4-methylcoumarin), one enzyme active against Z-Arg–AMC, several enzymes active against Leu–AMC, and (for the first time in plants) several enzymes active against Phe–AMC were identified.
COBISS.SI-ID: 3576952
Changes in gene expression in leaves of common bean at different levels of dehydration were identified by differential display reverse transcriptase PCR and confirmed by quantitative real-time PCR. The levels of 16 transcripts were changed significantly in all cultivars under both growth conditions, 9 being increased and seven decreased. The results of the studies form a basis for further studies of mechanisms of drougt resistance in plants.
COBISS.SI-ID: 2463336
Fourteen microsatellite markers were used to describe genetic diversity in a sample of 128 common bean accessions cultivated within the territory of Slovenia and its nearby regionsbetween 1800 and 2000. A total of 130 alleles were generated. In the UPGMA dendrogram, all studied accessions were intermixed in three main clusters, indicating that the diversity in the time periods overlapped. The analysis of molecular variance showed that a great part of genetic diversity has been preserved till today, confirming the results of cluster analysis.
COBISS.SI-ID: 2550888
The present work was conducted to evaluate AFLP (Amplified Fragment Length Polymorphism) and SSR (Simple Sequence Repeat) marker systems for their ability to detect genetic diversity within a set of 29 common bean accessions spanning both the Andean and Mesoamerican gene pools and to compare the efficiency of these two marker types in the classification of accessions according to the gene pools of origin.
COBISS.SI-ID: 5590905