J4-9476 — Annual report 2008
1.
Changes in levels of activity of serine proteases accompany the exposure of common bean (Phaseolus vulgaris L.) to water deficit.

A wide variety of proteolytic enzymes exist in plants. On their levels depends protein turnover, a fundamental component in plant development and adaptation to environmental conditions. Cysteine proteases have frequently been reported to be influenced by drought, but only a few serine proteases (SP). Our results point to a number of roles for different SPs in the plant response to water stress, which could range from enhanced protein turnover to limited proteolysis at specific sites.

B.03 Paper at an international scientific conference

COBISS.SI-ID: 2702696
2.
The expression profiles of selected genes in leaves of different beans species (Phaseolus spp.) under drought stress.

Relative gene expression analysis using qPCR showed that the mode of the expression of these transcripts is consistent among the four studied Phaseolus species. The results indicate that these transcripts are involved in the general response to drought stress. Promising results were obtained in our preliminary study using microarray technology and soybean GeneChip Array (Affymetrix). Almost 15% of the 35000 probe sets were identified which consistently detected transcripts in control and water-stressed plants of bean genotype Tiber and many of them showed different mode of expression.

B.03 Paper at an international scientific conference

COBISS.SI-ID: 2845544