Relative gene expression analysis using qPCR showed that the mode of the expression of these transcripts is consistent among the four studied Phaseolus species. The results indicate that these transcripts are involved in the general response to drought stress. Promising results were obtained in our preliminary study using microarray technology and soybean GeneChip Array (Affymetrix). Almost 15% of the 35000 probe sets were identified which consistently detected transcripts in control and water-stressed plants of bean genotype Tiber and many of them showed different mode of expression.
F.02 Acquisition of new scientific knowledge
COBISS.SI-ID: 2845544A wide variety of proteolytic enzymes exist in plants. Cysteine proteases have frequently been reported to be influenced by drought, but only a few serine proteases. Our results point to a number of roles for different SPs in the plant response to water stress.
F.02 Acquisition of new scientific knowledge
COBISS.SI-ID: 21158439The identification of genes whose expression is altered under conditions of drought is an important first step towards understanding the response of this species. Changes in gene expression in their leaves at different levels of dehydration were identified by differential display reverse transcriptase PCR and confirmed by quantitative real-time PCR. The levels of 15 transcripts were changed significantly (p<0.05) in all cultivars under both growth conditions, 8 being increased and seven decreased. Five of the genes identified have not previously been reported as being influenced by drought.
F.02 Acquisition of new scientific knowledge
COBISS.SI-ID: 2474344A wide variety of proteolytic enzymes exist in plants. On their levels depends protein turnover, a fundamental component in plant development and adaptation to environmental conditions. Cysteine proteases have frequently been reported to be influenced by drought, but only a few serine proteases (SP). Our results point to a number of roles for different SPs in the plant response to water stress, which could range from enhanced protein turnover to limited proteolysis at specific sites.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 2702696We developed a procedure, involving zymography with fluorescent substrates, which has revealed the heterogeneity of serine proteases and enabled us to detect and quantitate relative proteolytic activities in bean leaves. Levels of several serine proteases changed in different ways under water deficit. We isolated a serine protease (BSP) and a phenylalanyl aminopeptidase (FAP) involved in the response of P. vulgaris to drought and cloned bsp and fap genes. We studied the gene expression of BSP and FAP in leaf tissues of plants submitted to water deficit.
F.02 Acquisition of new scientific knowledge
COBISS.SI-ID: 3110760