We demonstrated for the first time that the electrophoresis is key for the efficient electrogene transfection in cases where plasmid DNA concentrations are relatively low (typically in vivo). In contrast, for in vitro the efficient electropermeabilization suffices for transfection. Presented results are essential for understanding of the mechanisms of gene electrotransfection and for improvement of the protocols. We also showed that the use of low DNA concentrations better represents in vivo conditions and allows better transfer of conclusions from in vitro to in vivo conditions.
COBISS.SI-ID: 6679380
We presented a series of experimental measurements of conductivity during and after electroporation and calculation of fraction of transient pores and long-lived stable pores in the cell membrane. We showed that the existing theoretical models, which describe formation of transient pores in the membrane, cannot describe long-lived pores that are key for successful electrogene transfection and electrotherapy. In article we calculate the fraction of the stable pores with respect to the electric field and number of pulses and show that larger number of pulses is vital for the pore stabilization.
COBISS.SI-ID: 6443604