We published a comparison of structure and mode of action of proteins that form pores in cellular membranes. Listeriolysin is a cholesterol dependent cytolysin, which share similar structure with MACPFdomains from complement and perforin. Understanding of LLO and CDC mechanism of pore formation will contribute to understanding of pore formation by important proteins from the immune system.
COBISS.SI-ID: 1893967
We published a preliminary study, which describes binding of LLO to lipid membranes. We also assessed factors that influence the binding. We employed various biophysical approaches such as surface plasmon resonance, lipid monolayers, fluorescence spectroscopy etc. We show that there is a limiting concentration of cholesterol that allows stable binding to the lipid membranes and that LLO can bind to lipid membranes even at suboptimal but physiological pH.
COBISS.SI-ID: 22569689
The method of surface plasmon resonance was used to monitor kinetics of cholesterol extraction by cyclodextrin. In this paper we developed the methods and used CDC streptolysin O as a proof that the membrane is devoid of cholesterol after treatment with cyclodextrin.
COBISS.SI-ID: 1826895
Cholesterol has an important structural role in lipid membranes. Here we descirbe how it is possible to monitor its extraction from lipid membranes by various biophysical methods. In particular we describe surface plasmon resonance approach, which was developed during this project.
Here we descirbe the use of surface plasmon resonance in basic sciences and various analytical approaches. We provide brief teoretical basis, how measurements are performed and can this method be used to study molecular interactions.
COBISS.SI-ID: 23308761