1.

Small protein gp6 of bacteriophage GIL01 induces litic cycle in bacteria Bacillus thuringiensis

As part of the research on this project, Anja Pavlin completed her master's thesis in which she proves that the small protein gp6 acts as a genetic switch of the virus to switch to the lytic cycle of the virus by binding to DNA. For this work Anja received the University Prešeren Award.

D.10 Educational activities

COBISS.SI-ID: 4890191

2.

Biological role of interaction of bacterial aegerolysins with lipids

As part of the research of this project, Eva Kočar completed her master's thesis in which she proves that the P. aeruginosa RaHU protein interacts with membrane of insect cells and enters the nucleus of the fixed human cells where it may interact with the DNA. For this work Eva received the Prešeren Award awarded by Biotechnical Faculty.

D.10 Educational activities

COBISS.SI-ID: 5068623

3.

Biotechnological applications of bacterial microcompartments

Bacterial microcompartments are protein organeles involved in various metabolic processes. In the process of development of the novel method, to elucidate nucleoprotein complexes, Jan Otoničar for his graduate thesis designed the protocol to encapsulate into the Pdu microcompartment a transcription factor LacI, which is fused to short peptide sequence of protein PduD, directing the protein into the compartment, and to enhanced green fluorescent protein (EGFP) at the amino- or the carboxy- terminus, respectively. The experimental setup enables to isolate the microcompartments by the affinity chromatography from E. coli cells. Results are fundamental for the project that we developed in the course of this project, enabling the encapsulation of a DNA fragment directly in bacterium. This approach has potential in biotechnology.

F.05 Ability to launch new technological development cycle

COBISS.SI-ID: 9282937

4.

Viral small proteins that control the bacterial cell fate

In the invited lecture, the we presented the results of the research and show the importance of small proteins on the regulation of transcription in bacteria.

B.04 Guest lecture

COBISS.SI-ID: 5184591

5.

The LexA regulatory system

Chapter was written for a broad, cross-disciplinary audience (also for students), and we describe the characteristics and function of the global bacterial transcription factor and illustrate how antibiotics modulate transcriptional responses and downstream processes in bacteria that can lead to drug resistance.

D.10 Educational activities

COBISS.SI-ID: 61057539