Transfer and segregation of immunoglobulins in the avian embryo

Based on our data from a pilot investigation we assume that the transfer of maternal antibodies of the IgA, IgG and IgM classes from the egg white into digestive tract of the avian embryo can differ considerably from the transfer already described in the literature. We wish to confirm experimentally that initially IgA, IgG and IgM antibodies from the egg white are transferred to the allantoic fluid (ALF). In addition, we would like to understand the role of the secretory component in the transfer of antibodies. Our investigations will further focus on the transfer of antibodies from the ALF to the embryonic bile where maternal antibodies reach titers 100 times higher than in the egg white. We assume that this project will reveal a new, biologically very important mechanism, enabling protection of the avian embryo against pathogenic organisms infecting the oviduct.