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Projects / Programmes
Development of immunoenzyme tests for the simultaneous detection of antibodies in mycoplasmal and viral infections of poultry
Research activity
| Code | Science | Field | Subfield |
|---|---|---|---|
| 4.06.03 | Biotechnical sciences | Biotechnology | Animal biotechnology |
| Code | Science | Field |
|---|---|---|
| B230 | Biomedical sciences | Microbiology, bacteriology, virology, mycology |
| B750 | Biomedical sciences | Veterinary medicine: surgery, physiology, pathology, clinical studies |
Keywords
veterinary medicine, diagnosis, antibody detection, poultry
Evaluation
(metodology)
Citations
Citations for bibliographic records in COBIB.SI that are linked to records in citation databases
Organisations (2) , Researchers (6)
0402 University of Ljubljana, Biotechnical Faculty, ZOO Technical Department
| no. | Code | Name and surname | Research area | Role | Period | No. of publicationsNo. of publications |
|---|---|---|---|---|---|---|
| 1. | 07914 | Veterinarian medicine | Head | 1998 - 2001 | 223 | |
| 2. | 05098 | Biotechnology | Researcher | 1998 - 2001 | 970 | |
| 3. | 02977 | Animal production | Researcher | 1998 - 2001 | 267 | |
| 4. | 05008 | Biotechnology | Researcher | 1999 - 2001 | 708 |
0406 University of Ljubljana, Veterinary Faculty
| no. | Code | Name and surname | Research area | Role | Period | No. of publicationsNo. of publications |
|---|---|---|---|---|---|---|
| 1. | 00911 | Veterinarian medicine | Researcher | 1999 - 2001 | 139 | |
| 2. | 08023 | Veterinarian medicine | Researcher | 1999 - 2001 | 436 |
Abstract
Immunoenzyme tests in the form of dot immunobinding assay (DIBA) enabling simultaneous detection of antibodies against Mycoplasma gallisepticum, M.synoviae, M.iowae and M.meleagridis as well as against Newcastle disease and avian influenza (AI) viruses in sera of examined chickens and turkeys will be developed. As specific antigens immunodominant membrane proteins of Mycoplasmas (pMGA, pMSB, etc) and immunodominant viral proteins fixed onto nitro cellulose or PVDF membranes will be used. DIBA tests will highly enhace the analytical capacity of currently used individual ELISA tests and significantly improve sensitivity and specificity of detecting of antibodies to pathogenic avian Mycoplasma species.