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Projects / Programmes
Obrambni proteini v rastlinskih celicah (Slovene)
Research activity
| Code | Science | Field | Subfield |
|---|---|---|---|
| 4.06.05 | Biotechnical sciences | Biotechnology | Plant biotechnology |
| Code | Science | Field |
|---|---|---|
| B191 | Biomedical sciences | Plant biochemistry |
| T490 | Technological sciences | Biotechnology |
Keywords
cDNA, PVYNTN, insects, virus, proteases, inhibitors, cloning, Agrobacterium, plant, potato, defence, engineering, Colorado potato beetle
Evaluation
(metodology)
Citations
Citations for bibliographic records in COBIB.SI that are linked to records in citation databases
Organisations (2) , Researchers (17)
0106 Jožef Stefan Institute
| no. | Code | Name and surname | Research area | Role | Period | No. of publicationsNo. of publications |
|---|---|---|---|---|---|---|
| 1. | 12688 | Biotechnology | Researcher | 1999 - 2001 | 1,041 | |
| 2. | 14804 | Biochemistry and molecular biology | Researcher | 1999 - 2001 | 123 | |
| 3. | 01084 | Biochemistry and molecular biology | Researcher | 1996 - 2001 | 193 | |
| 4. | 19170 | Pharmacy | Researcher | 1996 - 2001 | 157 | |
| 5. | 04360 | Biochemistry and molecular biology | Researcher | 2000 - 2001 | 137 | |
| 6. | 05266 | Biochemistry and molecular biology | Researcher | 1999 - 2001 | 115 | |
| 7. | 15813 | Neurobiology | Researcher | 1996 - 2001 | 441 | |
| 8. | 07849 | Biochemistry and molecular biology | Head | 1998 - 2001 | 1,148 |
0105 National Institute of Biology
| no. | Code | Name and surname | Research area | Role | Period | No. of publicationsNo. of publications |
|---|---|---|---|---|---|---|
| 1. | 18343 | Researcher | 1998 - 2001 | 53 | ||
| 2. | 08276 | Biotechnology | Researcher | 1998 - 2001 | 171 | |
| 3. | 04333 | Biotechnology | Researcher | 1998 - 2001 | 227 | |
| 4. | 18344 | Researcher | 1998 - 2001 | 43 | ||
| 5. | 10263 | Biotechnology | Researcher | 1996 - 2001 | 169 | |
| 6. | 18467 | Biotechnology | Researcher | 1996 - 2001 | 310 | |
| 7. | 05229 | Biotechnology | Researcher | 1998 - 2001 | 1,425 | |
| 8. | 15878 | Biotechnology | Researcher | 1996 - 2001 | 62 | |
| 9. | 03765 | Biotechnology | Researcher | 1998 - 2001 | 644 |
Abstract
Changes in expression of potato plant defence-related proteins, particulary proteinase inhibitors, as well as certain other pathogenesis-related proteins will be studied at the transcriptional and/or translational level before and after infection with PVYNTN virus, as well as before and after attack. by Colorado potato beetle. As important plant defense-proteins, known and novel proteinase inhibitors will be isolated, characterized and cloned. After testing for their inhibitory activity on PVY virus protease and some insect digestive proteinases, cDNAs corresponding to selected inhibitors will be inserted into potato genom by Agrobacterium-mediated gene transfer and transgenic plants will be generated and tested on the resistance against PVYNTN virus and Colorado potato beetle.