Bio-Layer Interferometry - BLI

BLI Octet R4 (Sartorius) enables the analysis of molecular interactions in real time without labelling the interaction partners. The system enables 4-channel monitoring of interactions (binding affinity and kinetics) between molecules larger than 150 Da, as well as determination of the concentration of biological molecules (e.g. proteins, peptides, DNA, RNA, etc.) in complex samples. The work is carried out in microtiter plates with 96 wells (requires at least 180 μL of sample) at a temperature between 15 and 40 °C. Biosensors are available for various methods of immobilization of one of the interaction partners (e.g. covalent binding via amine groups of the ligand, protein A for binding IgG, Ni-NTA for binding proteins marked with a hexahistidine sequence (His-tag), etc.).

Responsible for equipment:
The equipment is available at the Faculty of Pharmacy, Aškerčeva 7, 1000 Ljubljana. Possible access by agreement with the administrator. Due to the specificity of the equipment, the equipment administrator must be present at all times when working on the equipment.